Complementation of MC58cbbA with an ectopic copy of cbbA

In contrast steroids, rFBA with mutation(s) in the active (cation binding) site (D83A and H81A/H84A) had no detectable enzymatic activity.Employing flow cytometry, FBA could be detected on the surface of wild type N. Meningitidis MC58 cells but not on MC58cbbA (FBA deficient mutant). Complementation of MC58cbbA with an ectopic copy of cbbA (either wild type or D83A and H81A/H84A variants) restored the ability to express FBA on the surface suggesting the lack of involvement of the active site in the transportation of FBA to the cell surface.

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